Морфофункциональные изменения семенников у каланов в постнатальном онтогенезе

Abstract

A histological analysis of the testes, whose tissue samples were taken from fresh corpses of 18 sea otter aged from one month to two years in the period of their mass death on Bering Island in 1991-1995, was carried out. Testes were fixed in liquids of Shtiva, Buena. The material was embedded in paraffin. Sections 4-5µm thick were stained with hematoxylin-eosin, Heidengain’s azan, according to the method of trichrome and tetrachrome-CHIC. Morphometric characteristics were used as indicators of the development of spermatogenic epithelium: using a screw eyepiece micrometer, the diameter of the seminiferous tubules, the diameter of the nuclei of spermatogenic cells were measured, the area of the nuclei of sustentocytes and interstitial endocrinocytes was determined, and the number of stenttocytes in a single cross section of the seed canal was determined. Studies have revealed active spermatogenesis in the testicles of sea otter at the age of two years. In these sea otters, the diameter of the seminiferous tubules reaches a maximum; the convoluted seminiferous tubules contain all spermatogenic cells, including spermatozoa. Sustentocytes and interstitial endocrinocytes are characterized by signs of high functional activity.