The use of Levofloxacin and SUB-PRO in the treatment of bacterial haemorrhagic septicaemia in Cyprinids

The aim was to determine the effective dosages of Levofloxacin, an antibacterial drug new for aquaculture,   for the treatment of bacterial haemorrhagic septicaemia in carps and improvement of the physiological state of fish following disease and antibiotic therapy.

The methods: the experiments were carried out in aquaculture on juvenile carps infected with a mixed culture of pathogens: highly pathogenic strains of Aeromonas sp., Acinetobacter calcoaceticus, and Citrobacter. Three dosages of Levofloxacin were tested: 250, 500, and 1,000 mg/kg of ichtyomass with feed for 7 days. After the antibiotic therapy, a course of feeding was carried out with SUB-PRO probiotic at a dose of 0.4 g/kg of feed   for 10 days.

Scientific novelty: application of a broad-spectrum antibiotic Levofloxacin, an antibacterial drug new for aquaculture, in fish.

The results: positive results were obtained with Levofloxacin, an antibiotic new for aquaculture, in the treatment of bacterial haemorrhagic septicaemia. The advantage of the drug is the inhibitory effect on a wide range of bacterial agents and the absence of negative effects on the physiological state of fish. An effective dosage  of Levofloxacin (1,000 mg/kg of ichtyomass for 7 days) was established under experimental conditions. The positive dynamics on the blood parameters of the probiotic drug “SUB-PRO” after the disease and antibiotic therapy was revealed, which provided an increase in the resistance of the fish organism.

The practical significance: the findings will be crucial in the development of practical recommendations for  the treatment of bacterial haemorrhagic septicaemia of fish in aquaculture.

1. Amoros Jimenez G.C., Lartseva L.V. 1993. Intestinal microflora of commercial fish of the Volga-Caspian region // Abstr. Conf. of young scientists TINRO. «Biological and rational use of hydrobionts and their role in ecosystems». Vladivostok. Pp. 3–4.

2. Golovina N.A., Avdeeva E.V., Yevdokimova E.B., Kazimirchenko O.V., Kotlyarchuk M. Yu. 2016. Ichthyopathology manual. М.: Morkniga. 417 pp.

3. Ivanova N.T. 1983. Atlas of fish blood cells. Мoscow: Consumer goods and food industry. 184 pp.

4. Guidelines for haematology tests in fish. 1999. Instructions on the treatment of fish diseases. Part 2. Мoscow: Marketing department of AMB-agro. Pp. 69–97.

5. Romanova N. N., Golovin P. P., Yukhimenko L. N., Bolshakova D. S., Bolshakov S. V. 2020. The effect of SUB-PRO probiotic on the immunophysiological status of fish // Proc. res.-pract. conf. «Modern Problems of Veterinary Medicine, Animal Science, and Biotechnology», devoted to the 10th anniversary «Veterinary, Animal Science, and Biotechnology» Association. Мoscow: ZooVetKniga. Pp. 142–148.

6. Sidelnikova A. A., Nacheva L. V. 2015. Cytological features of the blood of rabbits in experimental opisthorchiasis // Modern problems of science and education. No. 2–3. https://science-education.ru/ru/article/view?id=2402811.08.2022.

7. Tarasova I. V. 2010. Basophils, mast cells, and thrombocytes as the immune and effector cells // Allergology and Immunology in Pediatrics. No. 1 (20). Pp. 32–36.

8. Shkurina Z. K., Printsevskaya V. A . 2003 Results of ichthyopathologic study of the Sakhalin pink and chum salmon spawners in 2002 // Trudy SakhNIRO. No. 5. Pp. 259–265.

9. Yukhimenko L. N., Bychkova L. I. 2007. Aetiological structure of causative agents of bacterial haemorrhagic septicaemia of fish // Problems of immunology, pathology, and health protection of fish and other aquatic organisms. Proc. of the inter. res.-pract. conf. Borok, 17–20 July 2007. Pp. 95–98.

10. Yukhimenko L. N., Tokareva S. B., Kukin M. S., Bychkova L. I. 2021. Medicinal and prophylactic measures against bacteriosis in fish farming // Veterinaria i Kormlenie. No. 6. Pp. 67–69 URL: https://doi.org/10.30917/ATTVK-1814–9588–2021–6–18

11. Atamas S., Chapoval S.P., Keegan A.D. 2013. Cytokines in chronic respiratory diseases // Biol. Rep. V. 5. № 3. Р. 1–12