Study of toxicity of arsenic-containing compounds isolated from brown algae Saccharina japonica in laboratory animals

The ability of various marine organisms, especially algae and invertebrates, to accumulate arsenic in high concentrations can pose a threat to public health when consumed. It is known from the literature that inorganic arsenic compounds (arsenites and arsenates) are the most toxic, in comparison with methylated forms of the element, and especially with complex organic compounds (arsenobetain, arsenocholine, tetramethylarsonium, arsenoriboses), which are considered non-toxic for live organisms. Monitoring of safety indicators of aquatic biological resources in the main commercial basins of the Russian Federation has shown that the most common excess of total arsenic content is characteristic for algae. According to TR CU 021/2011, the total arsenic content in algae should be 5 mg / kg and the established norm without separation of organic and inorganic arsenic compounds creates a barrier to the rational use of seafood. In this regard, the justification of the norms for the content of inorganic arsenic in algae and the assessment of their toxicity is a very urgent problem. Study of the samples of commercial brown algae Saccharina (=Laminaria) japonica and its derivates with ICP-MS, HPLC-MS-ISP methods, the maximum permissible level of arsenic was found to be exceeded, but the most toxic inorganic forms made up from 6 to14 % of the total amount of arsenic in the raw material. Acute toxicity on laboratory animals (rats) was studied and the absence of toxic effects was shown when an oral suspension containing high doses of arsenic was administered. Repeated administration of the same substances to laboratory mice of the CD-1 line has shown no toxic effects even after multiple doses of arsenic isolated from algae.

algae, inorganic arsenic compounds, acute toxicity, chronic toxicity, laboratory animals

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